In Vitro Evaluation of No-Carrier-Added Radiolabeled Cisplatin ([189, 191Pt]cisplatin) Emitting Auger Electrons
نویسندگان
چکیده
Due to their short-range (2–500 nm), Auger electrons (Auger e−) have the potential induce nano-scale physiochemical damage biomolecules. Although DNA is primary target of e−, it remains challenging maximize interaction between e− and DNA. To assess DNA-damaging effect released as close possible without chemical damage, we radio-synthesized no-carrier-added (n.c.a.) [189, 191Pt]cisplatin evaluated both its in vitro properties effect. Cellular uptake, intracellular distribution, binding were investigated, double-strand breaks (DSBs) by immunofluorescence staining γH2AX gel electrophoresis plasmid Approximately 20% radio-Pt was a nucleus, about 2% intra-nucleus bound DNA, although uptake n.c.a. radio-cisplatin low (0.6% incubated dose after 25-h incubation), resulting frequency cells with foci (1%). Nevertheless, some treated had aggregates unlike non-radioactive cisplatin. These findings suggest causes severe DSBs release very carriers. Efficient radio-drug delivery necessary for successful clinical application e−.
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2021
ISSN: ['1661-6596', '1422-0067']
DOI: https://doi.org/10.3390/ijms22094622